ARG65044
anti-GPR17 antibody
anti-GPR17 antibody for ICC/IF,Western blot and Human,Mouse
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GPR17 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat |
| Tested Application | ICC/IF, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GPR17 |
| Antigen Species | Human |
| Immunogen | C-EGKTNESSLSAKSE |
| Conjugation | Un-conjugated |
| Alternate Names | R12; P2Y-like receptor; G-protein coupled receptor 17; Uracil nucleotide/cysteinyl leukotriene receptor; UDP/CysLT receptor |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Mouse brain | ||||||
| Observed Size | ~ 41 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13304 Human Uracil nucleotide/cysteinyl leukotriene receptor Swiss-port # Q6NS65 Mouse Uracil nucleotide/cysteinyl leukotriene receptor |
|---|---|
| Gene Symbol | GPR17 |
| Gene Full Name | G protein-coupled receptor 17 |
| Function | Dual specificity receptor for uracil nucleotides and cysteinyl leukotrienes (CysLTs). Signals through G(i) and inhibition of adenylyl cyclase. May mediate brain damage by nucleotides and CysLTs following ischemia. [UniProt] |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 41 kDa |
Images (3) Click the Picture to Zoom In
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ARG65044 anti-GPR17 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG65044 anti-GPR17 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG65044 anti-GPR17 antibody WB image
Western blot: 35 µg of Mouse brain lysate (in RIPA buffer) stained with ARG65044 anti-GPR17 antibody at 3 µg/ml dilution and incubated at RT for 1 hour.
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ARG65044 anti-GPR17 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG65044 anti-GPR17 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
