ARG66561
anti-GPR143 / OA1 antibody
anti-GPR143 / OA1 antibody for ICC/IF and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GPR143 / OA1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GPR143 / OA1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from the internal region of Human GPR143. at AA rangle: 120-200 |
| Conjugation | Un-conjugated |
| Alternate Names | Ocular albinism type 1 protein; G-protein coupled receptor 143; NYS6; OA1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GPR143 |
| Gene Full Name | G protein-coupled receptor 143 |
| Background | This gene encodes a protein that binds to heterotrimeric G proteins and is targeted to melanosomes in pigment cells. This protein is thought to be involved in intracellular signal transduction mechanisms. Mutations in this gene cause ocular albinism type 1, also referred to as Nettleship-Falls type ocular albinism, a severe visual disorder. A related pseudogene has been identified on chromosome Y. [provided by RefSeq, Dec 2009] |
| Function | Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca(2+) influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand-dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport. [UniProt] |
| Cellular Localization | Melanosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Note=Distributed throughout the endo-melanosomal system but most of endogenous protein is localized in unpigmented stage II melanosomes. Its expression on the apical cell membrane is sensitive to tyrosine (PubMed:18828673). [UniProt] |
| Calculated MW | 44 kDa |
| PTM | Glycosylated. Phosphorylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG66561 anti-GPR143 / OA1 antibody ICC/IF image
Immunofluorescence: LOVO cells stained with ARG66561 anti-GPR143 / OA1 antibody. The picture on the right was blocked with the synthetic peptide.
