ARG64541

anti-GPR125 antibody

anti-GPR125 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Signaling Transduction antibody

Overview

Product Description

Goat Polyclonal antibody recognizes GPR125

Tested Reactivity Hu
Predict Reactivity Ms
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name GPR125
Antigen Species Human
Immunogen CTNKSASSFKNS
Conjugation Un-conjugated
Alternate Names PGR21; Adhesion G protein-coupled receptor A3; GPR125; TEM5L; G-protein coupled receptor 125

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.5 - 1.5 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 166647 Human ADGRA3

Swiss-port # Q8IWK6 Human Adhesion G protein-coupled receptor A3

Background This gene encodes a member of the G protein-coupled receptor superfamily. This membrane protein may play a role in tumor angiogenesis through its interaction with the human homolog of the Drosophila disc large tumor suppressor gene. This gene is mapped to a candidate region of chromosome 4 which may be associated with bipolar disorder and schizophrenia. [provided by RefSeq, Oct 2012]
Research Area Signaling Transduction antibody
Calculated MW 146 kDa

Images (2) Click the Picture to Zoom In

  • ARG64541 anti-GPR125 antibody WB image

    Western Blot: Human Ovary lysate (35 µg protein in RIPA buffer) stained with ARG64541 anti-GPR125 antibody at 0.5 µg/ml dilution.

  • ARG64541 anti-GPR125 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64541 anti-GPR125 antibody at 3.75 µg/ml dilution followed by AP-staining.