ARG10091
anti-GP IIb/IIIa antibody [237]
anti-GP IIb/IIIa antibody [237] for ELISA,Western blot and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [237] recognizes IGP IIb/IIIa |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 237 |
| Isotype | IgG1, kappa |
| Target Name | GP IIb/IIIa |
| Antigen Species | Human |
| Immunogen | Human platelet suspension |
| Conjugation | Un-conjugated |
| Alternate Names | GTA; GT; GPalpha IIb; PPP1R93; CD41; BDPLT2; BDPLT16; GP2B; Integrin alpha-IIb; GPIIb; Platelet membrane glycoprotein IIb; HPA3; CD antigen CD41; CD41B |
Application Instructions
| Application Note | ELISA: The mAb is reactive to platelet coated ELISA plate. Western Blot: The antibody reacts with GPIIb/IIIa on Western blot with platelet membrane glycoprotein extract. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | 0.01M PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGA2B |
| Gene Full Name | integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) |
| Background | Human platelet membrane glycoprotein Glycoprotein IIb/IIIa (GP IIb/IIIa) is a dominate receptor on the platelet membrane with very high molecular weight (>200kDa). After the platelet is activated, the GP IIb/IIIa receptor complex changes conformation and binds to fibrinogen in blood circulation with high affinity. The activation of platelets is initiated locally when the endothelial layer is wounded and the platelets are exposed to the collagen underneath the endothelium. The fibrinogen aggregates with platelets when binding to the GP IIb/IIIa receptor on platelets, thus primary haemostasis (Platelet clot) is formed. |
| Function | Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 113 kDa |
Clone References
Protein disulfide isomerase directly interacts with β-actin Cys374 and regulates cytoskeleton reorganization.
Effects of protein solution composition on the time-dependent functional activity of fibrinogen on surfaces.
Substantial changes of cellular iron homeostasis during megakaryocytic differentiation of K562 cells.
WB / Human
