ARG55054
anti-GOLPH3 antibody
anti-GOLPH3 antibody for ELISA,ICC/IF,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GOLPH3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GOLPH3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (14 aa) within the first 50 aa of Human GOLPH3. |
| Conjugation | Un-conjugated |
| Alternate Names | Mitochondrial DNA absence factor; Golgi phosphoprotein 3; GOPP1; Coat protein GPP34; MIDAS; Vps74; GPP34 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Rat Lung Tissue Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GOLPH3 |
| Gene Full Name | golgi phosphoprotein 3 (coat-protein) |
| Background | GOLPH3 Antibody: GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies. |
| Function | Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis. [UniProt] |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 34 kDa |
| PTM | Phosphorylated. |
Images (2) Click the Picture to Zoom In
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ARG55054 anti-GOLPH3 antibody WB image
Western blot: rat lung tissue lysate stained with ARG55054 anti-GOLPH3 antibody at (A) 0.5 and (B) 1 ug/ml dilution.
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ARG55054 anti-GOLPH3 antibody ICC/IF image
Immunofluorescence: Rat Lung cells stained with ARG55054 anti-GOLPH3 antibody at 20 ug/ml dilution.
