ARG58883

anti-GOLGA4 / Golgin 245 antibody

anti-GOLGA4 / Golgin 245 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes GOLGA4 / Golgin 245
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GOLGA4 / Golgin 245
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-150 of Human GOLGA4 / Golgin 245 (NP_002069.2).
Conjugation Un-conjugated
Alternate Names GCP2; CRPF46; Trans-Golgi p230; Golgin subfamily A member 4; GOLG; MU-RMS-40.18; p230; Golgin-245; 256 kDa golgin; Protein 72.1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MCF7
Observed Size 280 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2803 Human GOLGA4

GeneID: 54214 Mouse GOLGA4

Swiss-port # Q13439 Human Golgin subfamily A member 4

Swiss-port # Q91VW5 Mouse Golgin subfamily A member 4

Gene Symbol GOLGA4
Gene Full Name golgin A4
Background The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein has been postulated to play a role in Rab6-regulated membrane-tethering events in the Golgi apparatus. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010]
Function May play a role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with MACF1. [UniProt]
Cellular Localization Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, Golgi apparatus. [UniProt]
Calculated MW 261 kDa

Images (1) Click the Picture to Zoom In

  • ARG58883 anti-GOLGA4 / Golgin 245 antibody WB image

    Western blot: 25 µg of MCF7 cell lysate stained with ARG58883 anti-GOLGA4 / Golgin 245 antibody at 1:1000 dilution.