ARG57050
anti-GNMT antibody [5D1]
anti-GNMT antibody [5D1] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [5D1] recognizes GNMT |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5D1 |
| Isotype | IgG2a, kappa |
| Target Name | GNMT |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-295 of Human GNMT. |
| Conjugation | Un-conjugated |
| Alternate Names | Glycine N-methyltransferase; EC 2.1.1.20 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GNMT |
| Gene Full Name | glycine N-methyltransferase |
| Background | The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. The encoded protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). [provided by RefSeq, Oct 2008] |
| Function | Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. [UniProt] |
| Calculated MW | 33 kDa |
Images (2) Click the Picture to Zoom In
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ARG57050 anti-GNMT antibody [5D1] WB image
Western blot: 1) 50 ng of GNMT recombinant protein, 2) 40 µg of Liver tissue lysate stained with ARG57050 anti-GNMT antibody [5D1] at 1:1000.
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ARG57050 anti-GNMT antibody [5D1] WB image
Western blot: 10 µg of 1) 293T cell lysate, 2) GNMT Transfected 293T cell lysate stained with ARG57050 anti-GNMT antibody [5D1] at 1:1000.
