Overview

Product Description	Rabbit Polyclonal antibody recognizes GNE
Tested Reactivity	Hu, Rat
Tested Application	ICC/IF, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	GNE
Antigen Species	Human
Immunogen	Synthetic peptide of Human GNE.
Conjugation	Un-conjugated
Alternate Names	DMRV; GLCNE; ManAc kinase; IBM2; NM; Uae1; Uridine diphosphate-N-acetylglucosamine-2-epimerase; hydrolyzing; UDP-GlcNAc-2-epimerase; EC 3.2.1.183; EC 2.7.1.60; Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 IP 1:20 WB 1:1000 - 1:5000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HeLa
Observed Size	~ 75 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative	0.01% Sodium azide
Stabilizer	40% Glycerol and 0.05% BSA
Concentration	Batch dependent
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 10020 Human GNE GeneID: 114711 Rat GNE Swiss-port # O35826 Rat Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase Swiss-port # Q9Y223 Human Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinas
Gene Symbol	GNE
Gene Full Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Background	The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function	Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. [UniProt]
Cellular Localization	Cytoplasm. [UniProt]
Calculated MW	79 kDa
PTM	Phosphorylated by PKC. [UniProt]

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