ARG58908
anti-GM2A antibody
anti-GM2A antibody for Western blot and Rat
Overview
| Product Description | Goat Polyclonal antibody recognizes GM2A |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize isoform 1 (NP_000396.2) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GM2A |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from the internal region of Human GM2A. (NP_000396.2) (C-TTGNYRIESVLS) |
| Conjugation | Un-conjugated |
| Alternate Names | Ganglioside GM2 activator; SAP-3; Cerebroside sulfate activator protein; GM2-AP; Sphingolipid activator protein 3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | GM2A |
|---|---|
| Gene Full Name | GM2 ganglioside activator |
| Background | This gene encodes a small glycolipid transport protein which acts as a substrate specific co-factor for the lysosomal enzyme beta-hexosaminidase A. Beta-hexosaminidase A, together with GM2 ganglioside activator, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene result in GM2-gangliosidosis type AB or the AB variant of Tay-Sachs disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009] |
| Function | The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity (By similarity). Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. [UniProt] |
| Cellular Localization | Lysosome. [UniProt] |
| Calculated MW | 21 kDa |
| PTM | The serines in positions 32 and 33 are absent in 80% of the sequenced protein. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG58908 anti-GM2A antibody WB image
Western blot: 10 ug of HEK293 cell lysate (in RIPA buffer) overexpressing Human GM2A with DYKDDDDK tag stained with ARG58908 anti-GM2A antibody at 1 ug/ml dilution (Lane 1) and stained with anti-DYKDDDDK tag at 1:30000 dilution (Lane 3). Mock-transfected HEK293 stained with ARG58908 anti-GM2A antibody at 1 ug/ml dilution (Lane 2). Primary antibodies incubated at RT for 1 hour.
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ARG58908 anti-GM2A antibody WB image
Western blot: 35 µg of Rat kidney lysate (in RIPA buffer) stained with ARG58908 anti-GM2A antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
