ARG56692

anti-GM-CSF antibody

anti-GM-CSF antibody for ELISA,Neutralizing,Western blot and Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes GM-CSF
Tested Reactivity Rat
Tested Application ELISA, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GM-CSF
Antigen Species Rat
Immunogen E.coli derived Recombinant Rat GM-CSF.
(MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK)
Conjugation Un-conjugated
Alternate Names GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56801 as a detection antibody
Neut0.09 - 0.14 µg/ml (To yield [ND50] of the biological activity of Rat GM - CSF (0.5ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 116630 Rat CSF2

Swiss-port # P48750 Rat Granulocyte-macrophage colony-stimulating factor

Gene Symbol Csf2
Gene Full Name colony stimulating factor 2 (granulocyte-macrophage)
Background The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt]
Highlight Related products:
GM-CSF antibodies; GM-CSF ELISA Kits; GM-CSF Duos / Panels; GM-CSF recombinant proteins; Anti-Rabbit IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Calculated MW 16 kDa

Images (3) Click the Picture to Zoom In

  • ARG56692 anti-GM-CSF antibody WB image

    Western blot: 250 - 0.24 ng of Rat GM-CSF stained with ARG56692 anti-GM-CSF antibody, under reducing conditions.

  • ARG56692 anti-GM-CSF antibody WB image

    Western blot: 250 - 0.24 ng of Rat GM-CSF stained with ARG56692 anti-GM-CSF antibody, under non-reducing conditions.

  • ARG56692 anti-GM-CSF antibody standard curve image

    Sandwich ELISA: ARG56692 anti-GM-CSF antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56801 anti-GM-CSF antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.