ARG23251
anti-GM-CSF antibody [8D8]
anti-GM-CSF antibody [8D8] for ELISA,Western blot and Sheep
Overview
| Product Description | Mouse Monoclonal antibody [8D8] recognizes GM-CSF This antibody recognizes both recombinant and native ovine granulocyte / macrophage colony-stimulating factor (GM-CSF), also known as Colony stimulating factor (CSF). GM-CSF is one of several cytokines that regulate the survival, growth and differentiation of haemopoietic cells. GM-CSF is secreted from a range of cells including macrophages, T-cells, endothelial cells and fibroblasts in response to antigen, LPS or mitogen stimulation. This antibody has been reported to neutralize the activity of both recombinant and native ovine GM-CSF (Entrican et al. 1996). Removal of the preservative is recommended prior to use in functional assays. |
|---|---|
| Tested Reactivity | Sheep |
| Species Does Not React With | Bov |
| Tested Application | ELISA, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 8D8 |
| Isotype | IgG1 |
| Target Name | GM-CSF |
| Antigen Species | Sheep |
| Immunogen | CHO expressed recombinant ovine GM-CSF. |
| Conjugation | Un-conjugated |
| Alternate Names | GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CSF2 |
|---|---|
| Gene Full Name | colony stimulating factor 2 (granulocyte-macrophage) |
| Background | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt] |
| Highlight | Related products: GM-CSF antibodies; GM-CSF ELISA Kits; GM-CSF Duos / Panels; GM-CSF recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines |
| Calculated MW | 16 kDa |
