ARG10032
anti-GM-CSF antibody [59]
anti-GM-CSF antibody [59] for ELISA and Human
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [59] recognizes GM-CSF |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Does not show any cross reaction with other human cytokines or growth factors tested such as M-CSF, G-CSFR, IL-8, IL-16, IL-1β, TGF-β1 and TNF-α. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 59 |
| Isotype | IgG1, kappa |
| Target Name | GM-CSF |
| Antigen Species | Human |
| Immunogen | Purified recombinant human GM-CSF |
| Conjugation | Un-conjugated |
| Alternate Names | GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim |
Application Instructions
| Application Note | ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications for human GM-CSF detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated, Cat. No.: ARG10204). Suggested Capture Coating Dose: 0.3 μg/well; Substrate: TMB. If the above suggested conditions are followed approximately 2pg/mL of GM-CSF in serum/plasma or medium can be detected. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | 0.01M PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P04141 Human Granulocyte-macrophage colony-stimulating factor |
|---|---|
| Gene Symbol | CSF2 |
| Gene Full Name | colony stimulating factor 2 (granulocyte-macrophage) |
| Background | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30076 GM-CSF ELISA Antibody Duo Related products: GM-CSF antibodies; GM-CSF ELISA Kits; GM-CSF Duos / Panels; GM-CSF recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines |
| Research Area | Immune System antibody |
| Calculated MW | 16 kDa |
Clone References
Reduced BMPR2 expression induces GM-CSF translation and macrophage recruitment in humans and mice to exacerbate pulmonary hypertension.
