ARG65167
anti-GLUD1 + GLUD2 antibody
anti-GLUD1 + GLUD2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GLUD1 + GLUD2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody is expected to recognize both GLUD1 and GLUD2 proteins based on sequence homology analysis result. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GLUD1 + GLUD2 |
| Antigen Species | Human |
| Immunogen | C-ESEEQKRNRVRGILR |
| Conjugation | Un-conjugated |
| Alternate Names | GDH1; GDH 1; Glutamate dehydrogenase 1, mitochondrial; GDH; GLUD; EC 1.4.1.3; Glutamate dehydrogenase 2, mitochondrial; GLUDP1; EC 1.4.1.3; GDH 2; GDH2 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GLUD1 |
| Gene Full Name | glutamate dehydrogenase 1 |
| Background | This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans.[provided by RefSeq, Sep 2009] |
| Function | Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 61 kDa |
| PTM | ADP-ribosylated by SIRT4, leading to inactivate glutamate dehydrogenase activity (By similarity). Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer. |
Images (2) Click the Picture to Zoom In
-
ARG65167 anti-GLUD1 + GLUD2 antibody WB image
Western blot: 30 µg of HepG2 cell lysate stained with ARG65167 anti-GLUD1 + GLUD2 antibody at 1:500 dilution.
-
ARG65167 anti-GLUD1 + GLUD2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Cerebral Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65167 anti-GLUD1 + GLUD2 antibody at 3.8 µg/ml dilution followed by AP-staining.
Customer's Feedback
Excellent
Review for anti-GLUD1 + GLUD2 antibody
Application：WB
Sample：Rat liver
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-GLUD1 + GLUD2 antibody
Application：WB
Sample：Mouse liver
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
