ARG58881

anti-GLDC antibody

anti-GLDC antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes GLDC
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GLDC
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 36-290 of Human GLDC (NP_000161.2).
Conjugation Un-conjugated
Alternate Names GCE; GCSP; HYGN1; Glycine dehydrogenase (decarboxylating), mitochondrial; EC 1.4.4.2; Glycine cleavage system P protein; Glycine decarboxylase; Glycine dehydrogenase (aminomethyl-transferring)

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IPAssay-dependent
WB1:200 - 1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control DU145
Observed Size 113 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 104174 Mouse GLDC

GeneID: 2731 Human GLDC

Swiss-port # P23378 Human Glycine dehydrogenase (decarboxylating), mitochondrial

Swiss-port # Q91W43 Mouse Glycine dehydrogenase (decarboxylating), mitochondrial

Gene Symbol GLDC
Gene Full Name glycine dehydrogenase (decarboxylating)
Background Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).[provided by RefSeq, Jan 2010]
Function The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH). [UniProt]
Cellular Localization Mitochondrion,. [UniProt]
Calculated MW 113 kDa

Images (1) Click the Picture to Zoom In

  • ARG58881 anti-GLDC antibody WB image

    Western blot: 25 µg of DU145 cell lysate stained with ARG58881 anti-GLDC antibody at 1:1000 dilution.