ARG66430
anti-GIF / Gastric intrinsic factor antibody [SQab18119]
anti-GIF / Gastric intrinsic factor antibody [SQab18119] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Mouse Monoclonal antibody [SQab18119] recognizes GIF / Gastric intrinsic factor |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab18119 |
| Isotype | IgG1, kappa |
| Target Name | GIF / Gastric intrinsic factor |
| Antigen Species | Human |
| Immunogen | Recombinant Human GIF / Gastric intrinsic factor protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Gastric intrinsic factor; IFMH; TCN3; Intrinsic factor; INF; IF |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | Affinity purification with immunogen. |
|---|---|
| Buffer | PBS (pH 7.4) and 0.01% Thimerosal. |
| Preservative | 0.01% Thimerosal |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GIF |
| Gene Full Name | gastric intrinsic factor (vitamin B synthesis) |
| Background | This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq, Jul 2008] |
| Function | Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 45 kDa |
Images (4) Click the Picture to Zoom In
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ARG66430 anti-GIF / Gastric intrinsic factor antibody [SQab18119] ICC/IF image
Immunofluorescence: HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66430 anti-GIF / Gastric intrinsic factor antibody [SQab18119] (green) at 1:100 and cell nuclei were stained with Hoechst 33342 (blue).
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ARG66430 anti-GIF / Gastric intrinsic factor antibody [SQab18119] FACS image
Flow Cytometry: K562 cells were stained with ARG66430 anti-GIF / Gastric intrinsic factor antibody [SQab18119] at 2 µg/ml (red) and without antibody control (black).
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ARG66430 anti-GIF / Gastric intrinsic factor antibody [SQab18119] WB image
Western blot: 100 ng of Recombinant GIF protein, 30 µg of Mouse stomach lysate and 10 µl of Human gastric juice stained with ARG66430 anti-GIF / Gastric intrinsic factor antibody [SQab18119] at 1:5000 dilution.
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ARG66430 anti-GIF / Gastric intrinsic factor antibody [SQab18119] ELISA image
ELISA: Titration curve of ARG66430 anti-GIF / Gastric intrinsic factor antibody [SQab18119]. Red: GIF; Black: BSA (negative control).
