ARG65015
anti-GGCX antibody
anti-GGCX antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GGCX |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both isoforms (NP_000812.2; NP_001135741.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GGCX |
| Antigen Species | Human |
| Immunogen | C-PPESNPDPVHSE |
| Conjugation | Un-conjugated |
| Alternate Names | EC 4.1.1.90; Vitamin K gamma glutamyl carboxylase; Peptidyl-glutamate 4-carboxylase; Vitamin K-dependent gamma-carboxylase; Gamma-glutamyl carboxylase; VKCFD1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P38435 Human Vitamin K-dependent gamma-carboxylase |
|---|---|
| Background | This gene encodes an enzyme which catalyzes the posttranslational modification of vitamin K-dependent protein. Many of these vitamin K-dependent proteins are involved in coagulation so the function of the encoded enzyme is essential for hemostasis. Mutations in this gene are associated with vitamin K-dependent coagulation defect and PXE-like disorder with multiple coagulation factor deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2008] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 88 kDa |
Images (2) Click the Picture to Zoom In
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ARG65015 anti-GGCX antibody WB image
Western Blot: Human Muscle lysate (35 µg protein in RIPA buffer) stained with ARG65015 anti-GGCX antibody at 0.3 µg/ml dilution.
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ARG65015 anti-GGCX antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65015 anti-GGCX antibody at 5 µg/ml dilution followed by AP-staining.
