ARG55743

anti-GFP + YFP antibody

anti-GFP + YFP antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody
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Overview

Product Description Goat Polyclonal antibody recognizes GFP + YFP
Tested Reactivity Other
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Specificity This antibody recognizes YFP, GFP, EGFP and Venus from Aequorea victoria, and does not recognize mCherry/red fluorescent protein.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name GFP + YFP
Immunogen Purified recombinant GFP peptide from the jellyfish aequorea victoria
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50-1:1000
IHC-Fr1:50-1:1000
IHC-P1:50-1:1000
WB1:500-1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 3 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Images (2) Click the Picture to Zoom In

  • ARG55743 anti-GFP + YFP antibody ICC/IF image

    Immunocytochemistry: HEK293 cells transfected with YFP and stained with ARG55743 anti-GFP + YFP antibody at 1:50 dilution. Cells were fixed with 4% PFA.

  • ARG55743 anti-GFP + YFP antibody WB image

    Western blot: 50 µg of HEK293 cells transduced with YFP Ad and stained with ARG55743 anti-GFP + YFP antibody at 1:2000 dilution.

Specific References

SENP2 regulates mitochondrial function and insulin secretion in pancreatic β cells

WB / 

Jinyan Nan et al.
Exp Mol Med.,  (2022)

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