ARG58090
anti-GFM1 antibody
anti-GFM1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GFM1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GFM1 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 482-751 of Human GFM1 (NP_079272.4). |
| Conjugation | Un-conjugated |
| Alternate Names | EFG1; hEFG1; Elongation factor G, mitochondrial; Elongation factor G1; EGF1; EF-Gmt; mEF-G 1; GFM; COXPD1; Elongation factor G 1, mitochondrial; EFGM; EFG |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | H460 |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GFM1 |
| Gene Full Name | G elongation factor, mitochondrial 1 |
| Background | Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. [provided by RefSeq, Jul 2008] |
| Function | Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. [UniProt] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 83 kDa |
Images (2) Click the Picture to Zoom In
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ARG58090 anti-GFM1 antibody WB image
Western blot: 25 µg of H460 cell lysate stained with ARG58090 anti-GFM1 antibody at 1:3000 dilution.
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ARG58090 anti-GFM1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain stained with ARG58090 anti-GFM1 antibody at 1:100 dilution (40x lens).
