ARG66801
anti-GFAP antibody [SQab20213]
anti-GFAP antibody [SQab20213] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab20213] recognizes GFAP |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab20213 |
| Isotype | IgG |
| Target Name | GFAP |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 332-432 of Human GFAP. |
| Conjugation | Un-conjugated |
| Alternate Names | Glial fibrillary acidic protein; ALXDRD; GFAP |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GFAP |
| Gene Full Name | glial fibrillary acidic protein |
| Background | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Function | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
| Cellular Localization | Cytoplasm. Note=Associated with intermediate filaments. [UniProt] |
| Calculated MW | 50 kDa |
| PTM | Phosphorylated by PKN1. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG66801 anti-GFAP antibody [SQab20213] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human astrocytoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66801 anti-GFAP antibody [SQab20213].
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ARG66801 anti-GFAP antibody [SQab20213] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human brain tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66801 anti-GFAP antibody [SQab20213].
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ARG66801 anti-GFAP antibody [SQab20213] WB image
Western blot: Mouse Brain stained with ARG66801 anti-GFAP antibody [SQab20213] at 1:1000 dilution.
