ARG62992
anti-GFAP antibody [GF-02]
anti-GFAP antibody [GF-02] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Pig
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [GF-02] recognizes Glial Fibrillary Acidic Protein (GFAP) |
|---|---|
| Tested Reactivity | Hu, Pig |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | The clone GF-02 exclusively reacts with intact GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system; it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | GF-02 |
| Isotype | IgM |
| Target Name | GFAP |
| Antigen Species | Pig |
| Immunogen | Pellet of porcine brain cold-stable proteins after depolymerization of microtubules. |
| Conjugation | Un-conjugated |
| Alternate Names | Glial fibrillary acidic protein; ALXDRD; GFAP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by thiophilic adsorption-affinity chromatography and precipitation methods. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Function | GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
| Highlight | Related products: GFAP antibodies; GFAP Duos / Panels; Anti-Mouse IgM secondary antibodies; Related news: Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody |
| Calculated MW | 50 kDa |
| PTM | Phosphorylated by PKN1. |
Images (1) Click the Picture to Zoom In
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ARG62992 anti-GFAP antibody [GF-02] WB image
Western blot: Porcine brain lysate stained with ARG62992 anti-GFAP antibody [GF-02].
Lane 1: immunostaining with anti-GFAP antibody. Lane 2: immunostaining with Isotype mouse IgM control.
