ARG62991
anti-GFAP antibody [GF-01]
anti-GFAP antibody [GF-01] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Cat,Pig
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [GF-01] recognizes Glial Fibrillary Acidic Protein (GFAP) |
|---|---|
| Tested Reactivity | Hu, Cat, Pig |
| Species Does Not React With | Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Specificity | The clone GF-01 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | GF-01 |
| Isotype | IgG1 |
| Target Name | GFAP |
| Antigen Species | Pig |
| Immunogen | Pellet of porcine brain cold-stable proteins after depolymerization of microtubules. |
| Conjugation | Un-conjugated |
| Alternate Names | Glial fibrillary acidic protein; ALXDRD; GFAP |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: The clone GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-P: Human brain (cortex, cerebellum) |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Function | GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
| Highlight | Related products: GFAP antibodies; GFAP Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody |
| Calculated MW | 50 kDa |
| PTM | Phosphorylated by PKN1. |
Images (2) Click the Picture to Zoom In
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ARG62991 anti-GFAP antibody [GF-01] IHC-P image
Immunohistochemistry: Human cerebellum (paraffin-embedded sections) stained with ARG62991 anti-GFAP antibody [GF-01].
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ARG62991 anti-GFAP antibody [GF-01] IHC-P image
Immunohistochemistry: Human brain cortex (paraffin-embedded sections) stained with ARG62991 anti-GFAP antibody [GF-01].
