ARG59777
anti-GEMIN2 antibody
anti-GEMIN2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GEMIN2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GEMIN2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human GEMIN2. |
| Conjugation | Un-conjugated |
| Alternate Names | Survival of motor neuron protein-interacting protein 1; Component of gems 2; Gemin-2; SMN-interacting protein 1; Gem-associated protein 2; SIP1; SIP1-delta |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Jurkat | ||||||
| Observed Size | 35 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GEMIN2 |
| Gene Full Name | gem (nuclear organelle) associated protein 2 |
| Background | This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. [provided by RefSeq, Aug 2011] |
| Function | The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. [UniProt] |
| Cellular Localization | Nucleus, gem. Cytoplasm. Note=Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm. [UniProt] |
| Calculated MW | 32 kDa |
Images (3) Click the Picture to Zoom In
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ARG59777 anti-GEMIN2 antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG59777 anti-GEMIN2 antibody at 1:500 dilution.
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ARG59777 anti-GEMIN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer stained with ARG59777 anti-GEMIN2 antibody at 1:200 dilution.
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ARG59777 anti-GEMIN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer stained with ARG59777 anti-GEMIN2 antibody at 1:100 dilution.
