ARG10088
anti-GCPII / PSMA antibody [YPSMA-1]
anti-GCPII / PSMA antibody [YPSMA-1] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [YPSMA-1] recognizes GCPII / PSMA |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | Little or no cross-reactivity to benign prostate hyperplasia (BPH) or to normal prostatic tissue. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | YPSMA-1 |
| Isotype | IgG2b, kappa |
| Target Name | GCPII / PSMA |
| Antigen Species | Human |
| Immunogen | Crude membrane protein preparation from pooled prostate malignant carcinoma from China. |
| Conjugation | Un-conjugated |
| Alternate Names | FOLH1; Folate Hydrolase 1; NAALAD1; GCPII; PSMA; PSM; Glutamate Carboxypeptidase 2; GCP2; FOLH; N-Acetylated-Alpha-Linked Acidic Dipeptidase I; Pteroylpoly-Gamma-Glutamate Carboxypeptidase; Folylpoly-Gamma-Glutamate Carboxypeptidase; Cell Growth-Inhibiting Gene 27 Protein; Membrane Glutamate Carboxypeptidase; Glutamate Carboxypeptidase II; Glutamate Carboxylase II; EC 3.4.17.21; NAALADase I; NAALAdase; FGCP; MGCP; Folate Hydrolase (Prostate-Specific Membrane Antigen) 1; N-Acetylated Alpha-Linked Acidic Dipeptidase 1; Prostate-Specific Membrane Antigen |
Application Instructions
| Application Suggestion |
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| Application Note | Immuno-histochemistry: This antibody was used on formalin-fixed paraffin embedded and frozen tissue sections. Indirect ELISA: This antibody is reactive to PSMA expressing LNCap cells coated ELISA plate. Western blot：The antibody, when used at 1μg/ml concentration, identified the 100KDa PSMA in LNcap cell extract. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | 0.01M PBS (pH 7.0) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FOLH1 |
| Gene Full Name | folate hydrolase (prostate-specific membrane antigen) 1 |
| Background | This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. |
| Function | Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression. |
| Cellular Localization | Cell membrane, Cytoplasm, Membrane |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 84 kDa |
| PTM | Glycoprotein, Phosphoprotein |
Clone References
PSMA, EpCAM, VEGF and GRPR as imaging targets in locally recurrent prostate cancer after radiotherapy.
IHC-P / Human
Identification of a LNCaP-specific binding peptide using phage display.
WB / Human
