ARG53934
anti-GCPII / PSMA antibody [GCP-05]
anti-GCPII / PSMA antibody [GCP-05] for CyTOF®-candidate,Flow cytometry,ICC/IF,Immunoprecipitation and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [GCP-05] recognizes GCPII / PSMA |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | CyTOF®-candidate, FACS, ICC/IF, IP |
| Specificity | The clone GCP-05 recognizes extracellular domain of glutamate carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | GCP-05 |
| Isotype | IgG1 |
| Target Name | GCPII / PSMA |
| Antigen Species | Human |
| Immunogen | amino acids 44-750 of human GCPII |
| Conjugation | Un-conjugated |
| Alternate Names | FOLH1; Folate Hydrolase 1; NAALAD1; GCPII; PSMA; PSM; Glutamate Carboxypeptidase 2; GCP2; FOLH; N-Acetylated-Alpha-Linked Acidic Dipeptidase I; Pteroylpoly-Gamma-Glutamate Carboxypeptidase; Folylpoly-Gamma-Glutamate Carboxypeptidase; Cell Growth-Inhibiting Gene 27 Protein; Membrane Glutamate Carboxypeptidase; Glutamate Carboxypeptidase II; Glutamate Carboxylase II; EC 3.4.17.21; NAALADase I; NAALAdase; FGCP; MGCP; Folate Hydrolase (Prostate-Specific Membrane Antigen) 1; N-Acetylated Alpha-Linked Acidic Dipeptidase 1; Prostate-Specific Membrane Antigen |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FOLH1 |
| Gene Full Name | folate hydrolase (prostate-specific membrane antigen) 1 |
| Background | This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. |
| Function | Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression. |
| Cellular Localization | Cell membrane, Cytoplasm, Membrane |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 84 kDa |
| PTM | Glycoprotein, Phosphoprotein |
Images (2) Click the Picture to Zoom In
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ARG53934 anti-GCPII / PSMA antibody [GCP-05] FACS image
Flow Cytometry: LNCaP cells stained with ARG53934 anti-GCPII / PSMA antibody [GCP-05], followed by incubation with PE-labelled secondary antibody.
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ARG53934 anti-GCPII / PSMA antibody [GCP-05] CyTOF image
CyTOF: LNCaP cells were stained with ARG53934 anti-GCPII / PSMA antibody [GCP-05] (173Yb). Singlet cells were gated for data analysis.
Clone References
Gold nano-popcorn-based targeted diagnosis, nanotherapy treatment, and in situ monitoring of photothermal therapy response of prostate cancer cells using surface-enhanced Raman spectroscopy.
ICC/IF / Human
Expression of glutamate carboxypeptidase II in human brain.
Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding.
Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity.
Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II.
