ARG64397

anti-GCNT3 antibody

anti-GCNT3 antibody for Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes GCNT3
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name GCNT3
Antigen Species Human
Immunogen C-NKFDPKVDDNALQ
Conjugation Un-conjugated
Alternate Names C2GnT-mucin type; C2GNT2; GNTM; EC 2.4.1.150; C2/4GnT; hC2GnT-M; C24GNT; Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3; Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase; EC 2.4.1.102; C2GNTM; C2GnT-M

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9245 Human GCNT3

Swiss-port # O95395 Human Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminylt

Background This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009]
Research Area Signaling Transduction antibody
Calculated MW 51 kDa
PTM N-glycosylated.

Images (2) Click the Picture to Zoom In

  • ARG64397 anti-GCNT3 antibody WB image

    Western blot: Human Duodenum lysate (35 µg protein in RIPA buffer) stained with ARG64397 anti-GCNT3 antibody at 0.1 µg/ml dilution.

  • ARG64397 anti-GCNT3 antibody WB image

    Western blot: 1). Mock transfection; 2) GCNT3 (RC202007) expressing plasmid transfected HEK293 cell lysate stained with ARG64397 anti-GCNT3 antibody