ARG51039
anti-GATA1 antibody
anti-GATA1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Developmental Biology antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GATA1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GATA1 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around aa.140~144 (R-L-S-P-D) derived from Human GATA1. |
| Conjugation | Un-conjugated |
| Alternate Names | XLTDA; Eryf1; GATA-1; GF-1; GF1; NF-E1; ERYF1; XLANP; NFE1; GATA-binding factor 1; XLTT; NF-E1 DNA-binding protein; Erythroid transcription factor |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GATA1 |
| Gene Full Name | GATA binding protein 1 (globin transcription factor 1) |
| Background | GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. |
| Function | Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. [UniProt] |
| Research Area | Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 43 kDa |
| PTM | Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137 (By similarity). Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity (By similarity). Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo (By similarity). Acetylated on Lys-233, Lys-245 Lys-246 by EP300. |
Images (3) Click the Picture to Zoom In
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ARG51039 anti-GATA1 antibody WB image
Western blot: 20 µg of K562 and 3T3 cells stained with ARG51039 anti-GATA1 antibody at 1:500 dilution.
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ARG51039 anti-GATA1 antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-GATA1 antibody ARG51039 (left) or the same antibody preincubated with blocking peptide (right).
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ARG51039 anti-GATA1 antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with anti-GATA1 antibody ARG51039.
Customer's Feedback
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Review for anti-GATA1 antibody
Application：WB
Sample：K562 and 3T3
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
