ARG10735
anti-GAP43 antibody
anti-GAP43 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GAP43 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow, Hrs, Pig |
| Predict Reactivity | Chk |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GAP43 |
| Antigen Species | Mouse |
| Immunogen | C-terminal peptide of Mouse and Rat GAP43, which is KEDPEADQEHA, with an N-terminal Cys added to allow chemical coupling to KLH carrier protein. |
| Conjugation | Un-conjugated |
| Alternate Names | pp46; Growth-associated protein 43; B-50; Neuromodulin; PP46; Axonal membrane protein GAP-43; Neural phosphoprotein B-50 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Gap43 |
| Gene Full Name | growth associated protein 43 |
| Background | The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. |
Images (4) Click the Picture to Zoom In
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ARG10735 anti-GAP43 antibody ICC/IF image
Immunocytochemistry: Rat mixed neuron-glial cultures stained with ARG10735 anti-GAP43 antibody (red). Blue is DNA staining.
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ARG10735 anti-GAP43 antibody ICC/IF image
Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10735 anti-GAP43 antibody (green) at 1:2000 dilution and costained with Mouse mAb to vimentin (red) at 1:2000 dilution. DAPI (blue) for nuclear staining.
The GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.
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ARG10735 anti-GAP43 antibody WB image
Western blot: Homogenate of cow cerebellum stained with ARG10735 anti-GAP43 antibody.
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ARG10735 anti-GAP43 antibody WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord, SH-SY5Y and C6 cell lysates stained with ARG10735 anti-GAP43 antibody (green) at 1:20000 dilution.
The GAP43 protein is detected only in the lysates of neuronal origin. C6 cells are a Rat glioma cell line and do not express GAP43 protein.
