ARG10715
anti-GAP43 antibody [3H14]
anti-GAP43 antibody [3H14] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Overview
Product Description | Mouse Monoclonal antibody [3H14] recognizes GAP43 |
---|---|
Tested Reactivity | Hu, Ms, Rat |
Tested Application | ICC/IF, IHC-Fr, WB |
Host | Mouse |
Clonality | Monoclonal |
Clone | 3H14 |
Isotype | IgM |
Target Name | GAP43 |
Antigen Species | Human |
Immunogen | Recombinant full-length Human GAP43. |
Conjugation | Un-conjugated |
Alternate Names | pp46; Growth-associated protein 43; B-50; Neuromodulin; PP46; Axonal membrane protein GAP-43; Neural phosphoprotein B-50 |
Application Instructions
Application Suggestion |
|
||||||||
---|---|---|---|---|---|---|---|---|---|
Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
---|---|
Purification | Affinity purification. |
Buffer | PBS and 50% Glycerol. |
Stabilizer | 50% Glycerol |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
---|---|
Gene Symbol | GAP43 |
Gene Full Name | growth associated protein 43 |
Background | The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Function | This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. [UniProt] |
Calculated MW | 25 kDa |
PTM | Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. |
Images (4) Click the Picture to Zoom In
-
ARG10715 anti-GAP43 antibody [3H14] ICC/IF image
Immunocytochemistry: Mixed Rat neuronal cultures stained with ARG10715 anti-GAP43 antibody [3H14] (green). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.
-
ARG10715 anti-GAP43 antibody [3H14] ICC/IF image
Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10715 anti-GAP43 antibody [3H14] (red) at 1:1000 dilution and costained with ARG52328 anti-MAP2 antibody (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
The GAP43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons.
-
ARG10715 anti-GAP43 antibody [3H14] WB image
Western blot: 1) SH-SY5Y cell lysate, and 2) whole Rat brain lysate was stained with ARG10715 anti-GAP43 antibody [3H14] at 1: 2000 dilution.
-
ARG10715 anti-GAP43 antibody [3H14] WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord, C6 and SH-SY5Y cell lysates stained with ARG10715 anti-GAP43 antibody [3H14] (green) at 1:5000 dilution.
The protein is expressed in rodent and Human neurons and neuronal derived cells but not in C6 cells which are of glial origin.