ARG10714
anti-GAP43 antibody [1E3]
anti-GAP43 antibody [1E3] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig
Overview
| Product Description | Mouse Monoclonal antibody [1E3] recognizes GAP43 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow, Hrs, Pig |
| Predict Reactivity | Chk |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1E3 |
| Isotype | IgG1 |
| Target Name | GAP43 |
| Antigen Species | Mouse |
| Immunogen | C-terminal peptide of Mouse and Rat GAP43, which is KEDPEADQEHA, with an N-terminal Cys added to allow chemical coupling to KLH carrier protein. |
| Conjugation | Un-conjugated |
| Alternate Names | pp46; Growth-associated protein 43; B-50; Neuromodulin; PP46; Axonal membrane protein GAP-43; Neural phosphoprotein B-50 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Gap43 |
| Gene Full Name | growth associated protein 43 |
| Background | The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. |
Images (2) Click the Picture to Zoom In
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ARG10714 anti-GAP43 antibody [1E3] ICC/IF image
Immunocytochemistry: Mixed neuronal cultures stained with ARG10714 anti-GAP43 antibody [1E3] (green) and co-stained with rabbit antibody to MAP2 (red); DNA (blue). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.
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ARG10714 anti-GAP43 antibody [1E3] WB image
Western blot: Whole Rat spinal cord lysates stained with ARG10714 anti-GAP43 antibody [1E3]. Dots in middle of strips indicate position of 50 kDa and 37 kDa protein bands.
