ARG45633
anti-GALNT12 antibody
anti-GALNT12 antibody for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GALNT12 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GALNT12 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human GALNT12. |
| Conjugation | Un-conjugated |
| Alternate Names | GALNT12; Polypeptide N-Acetylgalactosaminyltransferase 12; GalNAc-T12; Polypeptide GalNAc Transferase 12; UDP-N-Acetyl-Alpha-D-Galactosamine:Polypeptide N-Acetylgalactosaminyltransferase 12 (GalNAc-T12); UDP-GalNAc:Polypeptide N-Acetylgalactosaminyltransferase 12; Protein-UDP Acetylgalactosaminyltransferase 12; Pp-GaNTase 12; EC 2.4.1.41 47; Colorectal Cancer, Susceptibility To 1; CRCS1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 67 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8IXK2 Human Polypeptide N-acetylgalactosaminyltransferase 12 |
|---|---|
| Gene Symbol | GALNT12 |
| Gene Full Name | Polypeptide N-Acetylgalactosaminyltransferase 12 |
| Background | This gene encodes a member of a family of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases, which catalyze the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to a serine or threonine residue on a polypeptide acceptor in the initial step of O-linked protein glycosylation. Mutations in this gene are associated with an increased susceptibility to colorectal cancer.[provided by RefSeq, Mar 2011] |
| Function | Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc-Muc5AC glycopeptide, but no detectable activity to mono-GalNAc-glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs. [UniProt] |
| Cellular Localization | Golgi apparatus; Membrane. [UniProt] |
| Calculated MW | 67 kDa |
| PTM | Disulfide bond. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG45633 anti-GALNT12 antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45633 anti-GALNT12 antibody at 5 μg/ml dilution.
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ARG45633 anti-GALNT12 antibody WB image
Western blot: K562 stained with ARG45633 anti-GALNT12 antibody at 0.5 μg/ml dilution.
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ARG45633 anti-GALNT12 antibody FACS image
Flow Cytometry: A549 stained with ARG45633 anti-GALNT12 antibody at 1 µg/10^6 cells dilution.
