ARG54931
anti-GADD45 alpha antibody
anti-GADD45 alpha antibody for Flow cytometry,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GADD45A |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Bov |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GADD45 alpha |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human GADD45 alpha. |
| Conjugation | Un-conjugated |
| Alternate Names | Growth arrest and DNA damage-inducible protein GADD45 alpha; DDIT1; GADD45; DNA damage-inducible transcript 1 protein; DDIT-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Y79 |
Properties
| Form | Liquid |
|---|---|
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P24522 Human Growth arrest and DNA damage-inducible protein GADD45 alpha |
|---|---|
| Gene Symbol | GADD45A |
| Gene Full Name | growth arrest and DNA-damage-inducible, alpha |
| Background | This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010] |
| Function | In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. [UniProt] |
| Cellular Localization | Nucleus. |
| Research Area | Gene Regulation antibody |
| Calculated MW | 18 kDa |
Images (2) Click the Picture to Zoom In
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ARG54931 anti-GADD45 alpha antibody WB image
Western blot: 35 µg of Y79 cell lysate stained with ARG54931 anti-GADD45 alpha antibody.
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ARG54931 anti-GADD45 alpha antibody FACS image
Flow Cytometry: K562 cells stained with ARG54931 anti-GADD45 alpha antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
