ARG64797
anti-GAD67 antibody
anti-GAD67 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GAD67 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GAD67 |
| Antigen Species | Human |
| Immunogen | C-PDSPQRREKLHK |
| Conjugation | Un-conjugated |
| Alternate Names | EC 4.1.1.15; Glutamate decarboxylase 67 kDa isoform; Glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD; SCP; GAD-67; CPSQ1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008] |
| Research Area | Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 67 kDa |
Images (2) Click the Picture to Zoom In
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ARG64797 anti-GAD67 antibody WB image
Western blot: 35 µg of Human brain (cerebral cortex) lysate stained with ARG64797 anti-GAD67 antibody at 0.5 µg/ml dilution.
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ARG64797 anti-GAD67 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64797 anti-GAD67 antibody at 4 µg/ml dilution followed by AP-staining.
