ARG42654
anti-GAD25 / GAD67 antibody
anti-GAD25 / GAD67 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GAD25 / GAD67 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GAD25 / GAD67 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-200 of Human GAD1 (NP_000808.2). |
| Conjugation | Un-conjugated |
| Alternate Names | EC 4.1.1.15; Glutamate decarboxylase 67 kDa isoform; Glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD; SCP; GAD-67; CPSQ1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Jurkat | ||||||||
| Observed Size | ~ 67 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GAD1 |
| Gene Full Name | glutamate decarboxylase 1 (brain, 67kDa) |
| Background | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the production of GABA. [UniProt] |
| Calculated MW | 67 kDa (GAD67) |
Images (3) Click the Picture to Zoom In
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ARG42654 anti-GAD25 / GAD67 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG42654 anti-GAD25 / GAD67 antibody at 1:100 dilution.
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ARG42654 anti-GAD25 / GAD67 antibody IHC image
Immunohistochemistry: Rat eyeball tissue stained with ARG42654 anti-GAD25 / GAD67 antibody at 1:100 dilution.
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ARG42654 anti-GAD25 / GAD67 antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG42654 anti-GAD25 / GAD67 antibody at 1:1000 dilution.
