ARG63756
anti-GAD2 / GAD65 antibody
anti-GAD2 / GAD65 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Metabolism antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GAD2 / GAD65 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported variants. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GAD2 / GAD65 |
| Antigen Species | Human |
| Immunogen | C-TLEDNEERMSRLSK |
| Conjugation | Un-conjugated |
| Alternate Names | Glutamate decarboxylase 65 kDa isoform; EC 4.1.1.15; GAD65; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008] |
| Research Area | Metabolism antibody; Neuroscience antibody |
| Calculated MW | 65 kDa |
| PTM | Phosphorylated; which does not affect kinetic parameters or subcellular location. Palmitoylated; which is required for presynaptic clustering. |
Images (3) Click the Picture to Zoom In
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ARG63756 anti-GAD2 / GAD65 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63756 anti-GAD2 / GAD65 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63756 anti-GAD2 / GAD65 antibody WB image
Western blot: 35 µg of Human brain (hippocampus) lysate (in RIPA buffer) stained with ARG63756 anti-GAD2 / GAD65 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
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ARG63756 anti-GAD2 / GAD65 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63756 anti-GAD2 / GAD65 antibody at 2.5 µg/ml dilution followed by AP-staining.
