ARG64228
anti-GABPB2 antibody
anti-GABPB2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GABPB2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Dog |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | This antibody is expected to recognise isoform beta 1 (NP_005245.2) and isoform gamma 1 (NP_002032.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GABPB2 |
| Antigen Species | Human |
| Immunogen | C-SSENSSKATDETG |
| Conjugation | Un-conjugated |
| Alternate Names | GABPB2; Transcription factor E4TF1-47; BABPB2; GABP subunit beta-1; GABP subunit beta-2; NRF2B1; NRF2B2; Transcription factor E4TF1-53; E4TF1; GA-binding protein subunit beta-1; Nuclear respiratory factor 2; E4TF1-47; GABPB; E4TF1B; GABPB-1; GABPB-2; E4TF1-53 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 42 kDa |
Images (6) Click the Picture to Zoom In
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ARG64228 anti-GABPB2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG64228 anti-GABPB2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64228 anti-GABPB2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64228 anti-GABPB2 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64228 anti-GABPB2 antibody WB image
Western blot: 35 µg of HeLa cell lysate (in RIPA buffer) with (B) and without (A) blocking peptide. The blots were stained with ARG64228 anti-GABPB2 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG64228 anti-GABPB2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG64228 anti-GABPB2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG64228 anti-GABPB2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64228 anti-GABPB2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64228 anti-GABPB2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64228 anti-GABPB2 antibody at 3.75 µg/ml dilution followed by AP-staining.
