ARG41479
anti-GABARAP antibody
anti-GABARAP antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GABARAP |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GABARAP |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 1-100 of Human GABARAP (NP_009209.1). |
| Conjugation | Un-conjugated |
| Alternate Names | A; MM46; GABARAP-a; Gamma-aminobutyric acid receptor-associated protein; ATG8A; GABA |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa | ||||||
| Observed Size | 15 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GABARAP |
| Gene Full Name | GABA(A) receptor-associated protein |
| Background | Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008] |
| Function | Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. [UniProt] |
| Cellular Localization | Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Cytoplasmic vesicle. Note=Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity). [UniProt] |
| Calculated MW | 14 kDa |
| PTM | The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG41479 anti-GABARAP antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG41479 anti-GABARAP antibody at 1:100 dilution.
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ARG41479 anti-GABARAP antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG41479 anti-GABARAP antibody at 1:1000 dilution.
Specific References
Slc20a1 and Slc20a2 Regulate Neuronal Plasticity and Cognition Independently of Their Phosphate Transport Ability
IHC-P/WB / Mouse
