ARG52304
anti-GABAB Receptor 2 phospho (Ser783) antibody
anti-GABAB Receptor 2 phospho (Ser783) antibody for ICC/IF,Western blot and Rat
Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GABAB Receptor 2 phospho (Ser783) |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Bov, Chk, Dog, NHuPrm |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GABAB Receptor 2 |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser783 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | HRIHFB2099; GABA-B receptor 2; GABA-B-R2; GPRC3B; GABA-BR2; G-protein coupled receptor 51; GPR51; GABABR2; Gamma-aminobutyric acid type B receptor subunit 2; HG20; Gb2 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for ~102k GABAB R2 phosphorylated at Ser783. Immunolabeling of the GABAB R2 band is completely blocked by λ-phosphatase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O88871 Rat Gamma-aminobutyric acid type B receptor subunit 2 |
|---|---|
| Gene Symbol | GABBR2 |
| Gene Full Name | gamma-aminobutyric acid (GABA) B receptor 2 |
| Background | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007). |
| Research Area | Neuroscience antibody |
| Calculated MW | 106 kDa |
Images (1) Click the Picture to Zoom In
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ARG52304 anti-GABAB Receptor 2 phospho (Ser783) antibody WB image
Western blot: Rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control) stained with ARG52304 anti-GABAB Receptor 2 phospho (Ser783) antibody. The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase).
