ARG22242
anti-GABAB Receptor 2 antibody [S81-2]
anti-GABAB Receptor 2 antibody [S81-2] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [S81-2] recognizes GABAB Receptor 2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Detects ~105kDa. No cross-reactivity against GABA(B)R1. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S81-2 |
| Isotype | IgG1 |
| Target Name | GABAB Receptor 2 |
| Antigen Species | Rat |
| Immunogen | Fusion protein around aa. 861-912 of Rat GABA B Receptor 2 |
| Conjugation | Un-conjugated |
| Alternate Names | HRIHFB2099; GABA-B receptor 2; GABA-B-R2; GPRC3B; GABA-BR2; G-protein coupled receptor 51; GPR51; GABABR2; Gamma-aminobutyric acid type B receptor subunit 2; HG20; Gb2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Gabbr2 |
| Gene Full Name | gamma-aminobutyric acid (GABA) B receptor 2 |
| Background | The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.[provided by RefSeq, Jan 2010] |
| Function | Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. [UniProt] |
| Cellular Localization | Cell Junction, Cell membrane, postsynaptic cell membrane, Synapse |
| Calculated MW | 106 kDa |
Images (2) Click the Picture to Zoom In
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ARG22242 anti-GABAB Receptor 2 antibody [S81-2] ICC/IF image
Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary antibody: ARG22242 anti-GABAB Receptor 2 antibody [S81-2] at 1:100 for 60 min at RT. Secondary antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Primary antibody (D) Composite.
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ARG22242 anti-GABAB Receptor 2 antibody [S81-2] WB image
Western blot: 10 µg of Rat Brain Membrane. Block: 5% milk. Primary antibody: ARG22242 anti-GABAB Receptor 2 antibody [S81-2] at 1:1000 for 1 hour at RT. Secondary antibody: Goat anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT.
