ARG52303
anti-GABAA Receptor gamma 2 phospho (Ser327) antibody
anti-GABAA Receptor gamma 2 phospho (Ser327) antibody for Western blot and Rat
Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GABAA Receptor gamma 2 phospho (Ser327) |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GABAA Receptor gamma 2 |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | A; CAE2; ECA2; GEFSP3; Gamma-aminobutyric acid receptor subunit gamma-2; GABA |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for ~45k GABAA receptor γ2 subunit phosphorylated at Ser327. Immunolabeling of the GABAA band is completely blocked by λ-phosphatase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P18508 Rat Gamma-aminobutyric acid receptor subunit gamma-2 |
|---|---|
| Gene Symbol | GABRG2 |
| Gene Full Name | gamma-aminobutyric acid (GABA) A receptor, gamma 2 |
| Background | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. It has recently been suggested that PKCΕ regulates the sensitivity of GABAA α1β2γ2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of γ2 (Qi et al., 2007) |
| Research Area | Neuroscience antibody |
| Calculated MW | 54 kDa |
| PTM | Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability. |
Images (1) Click the Picture to Zoom In
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ARG52303 anti-GABAA Receptor gamma 2 phospho (Ser327) antibody WB image
Western blot: Rat cortex showing specific immunolabeling of the ~45 kDa GABA γ 2 protein phosphorylated at Ser327 (control) stained with ARG52303 anti-GABAA Receptor gamma 2 phospho (Ser327) antibody. Immunolabeling is blocked by the phosphopeptide (Peptide) used as antigen but not by the corresponding dephosphopeptide.
