ARG52301
anti-GABAA Receptor gamma 2 antibody
anti-GABAA Receptor gamma 2 antibody for Immunohistochemistry,Western blot and Rat
Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GABAA Receptor gamma 2 |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Chk, NHuPrm, Zfsh |
| Tested Application | IHC, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GABAA Receptor gamma 2 |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide corresponding to amino acid residues specific to the gamma 2 subunit conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | A; CAE2; ECA2; GEFSP3; Gamma-aminobutyric acid receptor subunit gamma-2; GABA |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~46k γ2-subunit of the GABAA receptor in Western blots of Rat brain extracts. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Neat Serum |
| Buffer | Neat serum |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P18508 Rat Gamma-aminobutyric acid receptor subunit gamma-2 |
|---|---|
| Gene Symbol | GABRG2 |
| Gene Full Name | gamma-aminobutyric acid (GABA) A receptor, gamma 2 |
| Background | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, co-expression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of β-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003). |
| Research Area | Neuroscience antibody |
| Calculated MW | 54 kDa |
| PTM | Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability. |
Images (2) Click the Picture to Zoom In
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ARG52301 anti-GABAA Receptor gamma 2 antibody WB image
Western blot: 10 μg of rat hippocampal lysate stained with ARG52301 anti-GABAA Receptor gamma 2 antibody showing immunolabeling of the ~46k γ2-subunit of the GABAA-R.
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ARG52301 anti-GABAA Receptor gamma 2 antibody IHC image
Immunohistochemistry: rat cerebellum stained with ARG52301 anti-GABAA Receptor gamma 2 antibody showing labeling of GABAA g2 subunit in fuchsia.
