ARG24127
anti-GABAA Receptor beta 3 antibody [N87/25]
anti-GABAA Receptor beta 3 antibody [N87/25] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [N87/25] recognizes GABAA Receptor beta 3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | N87/25 |
| Isotype | IgG1 |
| Target Name | GABAA Receptor beta 3 |
| Antigen Species | Mouse |
| Immunogen | Fusion protein of Mouse GABAA Receptor beta 3 |
| Conjugation | Un-conjugated |
| Alternate Names | Gamma-aminobutyric acid receptor subunit beta-3; A; ECA5; GABA |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~53k β3-subunit of the GABAA receptor in Western blots. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GABRB3 |
| Gene Full Name | gamma-aminobutyric acid (GABA) A receptor, beta 3 |
| Background | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). |
| Research Area | Neuroscience antibody |
| Calculated MW | 54 kDa |
Images (3) Click the Picture to Zoom In
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ARG24127 anti-GABAA Receptor beta 3 antibody [N87/25] IHC-P image
Immunohistochemistry: Mouse Backskin stained with ARG24127 anti-GABAA Receptor beta 3 antibody [N87/25] at 1:1000 dilution.
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ARG24127 anti-GABAA Receptor beta 3 antibody [N87/25] ICC/IF image
Immunofluorescence: SH-SY5Y stained with ARG24127 anti-GABAA Receptor beta 3 antibody [N87/25] at 1:100 dilution.
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ARG24127 anti-GABAA Receptor beta 3 antibody [N87/25] WB image
Western blot: Rat brain stained with ARG24127 anti-GABAA Receptor beta 3 antibody [N87/25] at 1:1000 dilution.
