ARG64825
anti-GABAA Receptor alpha 4 antibody
anti-GABAA Receptor alpha 4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GABAA Receptor alpha 4 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GABAA Receptor alpha 4 |
| Antigen Species | Human |
| Immunogen | C-EKAKRKTSKPPQE |
| Conjugation | Un-conjugated |
| Alternate Names | A; Gamma-aminobutyric acid receptor subunit alpha-4; GABA |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P48169 Human Gamma-aminobutyric acid receptor subunit alpha-4 |
|---|---|
| Background | Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. This gene encodes subunit alpha-4, which is involved in the etiology of autism and eventually increases autism risk through interaction with another subunit, gamma-aminobutyric acid receptor beta-1 (GABRB1). Alternatively spliced transcript variants encoding different isoforms have been found in this gene.[provided by RefSeq, Feb 2011] |
| Research Area | Neuroscience antibody |
| Calculated MW | 62 kDa |
Images (2) Click the Picture to Zoom In
-
ARG64825 anti-GABAA Receptor alpha 4 antibody WB image
Western blot: Human Frontal Cortex lysate (35 µg protein in RIPA buffer) stained with ARG64825 anti-GABAA Receptor alpha 4 antibody at 0.1 µg/ml dilution.
-
ARG64825 anti-GABAA Receptor alpha 4 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64825 anti-GABAA Receptor alpha 4 antibody at 2.5 µg/ml dilution followed by AP-staining.
