ARG52287
anti-GABAA Receptor alpha 1 antibody
anti-GABAA Receptor alpha 1 antibody for Western blot and Mouse,Rat
Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GABAA Receptor alpha 1 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Predict Reactivity | Hu, Bov, Dog, NHuPrm |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GABAA Receptor alpha 1 |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | A; EJM; EIEE19; EJM5; Gamma-aminobutyric acid receptor subunit alpha-1; ECA4; GABA |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | Specific for the ~51k α1-subunit of the GABAA receptor in Western blots of Rat brain. Labeling is absent in α1-subunit knockout animals. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P62812 Mouse Gamma-aminobutyric acid receptor subunit alpha-1 Swiss-port # P62813 Rat Gamma-aminobutyric acid receptor subunit alpha-1 |
|---|---|
| Gene Symbol | GABRA1 |
| Gene Full Name | gamma-aminobutyric acid (GABA) A receptor, alpha 1 |
| Background | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzo-diazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). |
| Research Area | Neuroscience antibody |
| Calculated MW | 52 kDa |
Images (1) Click the Picture to Zoom In
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ARG52287 anti-GABAA Receptor alpha 1 antibody WB image
Western blot: 5-7 µg of Mouse cerebellum lysates from wild type (control) and alpha 1 knockout (alpha 1 K/O) animals showing specific immunolabeling of the ~51k alpha 1-subunit of the GABAA-R in the wild type but not in the alpha 1 K/O animals when stained with ARG52287 anti-GABAA Receptor alpha 1 antibody.
