ARG64254
anti-GAB2 antibody
anti-GAB2 antibody for Western blot and Human
Cancer antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GAB2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_536739.1; NP_036428.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GAB2 |
| Antigen Species | Human |
| Immunogen | C-QVDKEKTQALQN |
| Conjugation | Un-conjugated |
| Alternate Names | GRB2-associated binder 2; Growth factor receptor bound protein 2-associated protein 2; GRB2-associated-binding protein 2; pp100 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2009] |
| Research Area | Cancer antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 74 kDa |
| PTM | Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling. Dephosphorylated by PTPN11. |
Images (1) Click the Picture to Zoom In
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ARG64254 anti-GAB2 antibody WB image
Western Blot: K562 cell lysate (35 µg protein in RIPA buffer) stained with ARG64254 anti-GAB2 antibody at 0.5 µg/ml dilution.
