ARG51585
anti-G3BP1 phospho (Ser232) antibody
anti-G3BP1 phospho (Ser232) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes G3BP1 phospho (Ser232) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | G3BP1 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1. |
| Conjugation | Un-conjugated |
| Alternate Names | G3BP; hDH VIII; G3BP-1; GAP SH3 domain-binding protein 1; EC 3.6.4.13; EC 3.6.4.12; HDH-VIII; ATP-dependent DNA helicase VIII; Ras GTPase-activating protein-binding protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13283 Human Ras GTPase-activating protein-binding protein 1 |
|---|---|
| Gene Symbol | G3BP1 |
| Gene Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
| Background | May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. |
| Function | May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 52 kDa |
| PTM | Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization. Arg-435 is dimethylated, probably to asymmetric dimethylarginine. |
Images (3) Click the Picture to Zoom In
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ARG51585 anti-G3BP1 phospho (Ser232) antibody WB image
Western blot: Extracts from 293 cells untreated or treated with starvation stained with ARG51585 anti-G3BP1 phospho (Ser232) antibody.
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ARG51585 anti-G3BP1 phospho (Ser232) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51585 anti-G3BP1 phospho (Ser232) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51585 anti-G3BP1 phospho (Ser232) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51585 anti-G3BP1 phospho (Ser232) antibody.
