ARG63524
anti-Fyn antibody
anti-Fyn antibody for Western blot and Human
Cancer antibody; Neuroscience antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Fyn |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise all three reported isoforms (as represented by NP_002028.1; NP_694592.1; NP_694593.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Fyn |
| Antigen Species | Human |
| Immunogen | VQCKDKEATKLTE |
| Conjugation | Un-conjugated |
| Alternate Names | p59-FYN; Tyrosine-protein kinase Fyn; Src-like kinase; Proto-oncogene c-Fyn; p59-Fyn; Proto-oncogene Syn; SYN; SLK; EC 2.7.10.2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Neuroscience antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody |
| Calculated MW | 61 kDa |
| PTM | Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state (By similarity). PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling. Palmitoylation at Cys-3 and Cys-6 regulates subcellular location. |
Images (2) Click the Picture to Zoom In
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ARG63524 anti-Fyn antibody WB image
Western blot: Jurkat lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63524 anti-Fyn antibody at 0.2 µg/ml dilution.
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ARG63524 anti-Fyn antibody WB image
Western blot: 35 µg of A431 (A) and Jurkat (B) cell lysates (in RIPA buffer) stained with ARG63524 anti-Fyn antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
