ARG54079

anti-Fyn antibody

anti-Fyn antibody for ICC/IF,Western blot and Human,Mouse,Rat,Monkey

Cancer antibody; Neuroscience antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody

Overview

Product Description Mouse Monoclonal antibody recognizes FYN
Tested Reactivity Hu, Ms, Rat, Mk
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Target Name Fyn
Antigen Species Human
Immunogen Purified recombinant human Fyn protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names p59-FYN; Tyrosine-protein kinase Fyn; Src-like kinase; Proto-oncogene c-Fyn; p59-Fyn; Proto-oncogene Syn; SYN; SLK; EC 2.7.10.2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 59 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 10 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14360 Mouse FYN

GeneID: 25150 Rat FYN

GeneID: 2534 Human FYN

Gene Symbol FYN
Gene Full Name FYN proto-oncogene, Src family tyrosine kinase
Background Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival,cell adhesion,integrin-mediated signaling,cytoskeletal remodeling,cell motility,immune response and axon guidance.Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain.Following activation by PKA,the protein subsequently associates with PTK2/FAK1,allowing PTK2/FAK1 phosphorylation,activation and targeting to focal adhesions.Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT.Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage.Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1,KIRREL and TRPC6.Plays a role in neural processes by phosphorylating DPYSL2,a multifunctional adapter protein within the central nervous system,ARHGAP32,a regulator for Rho family GTPases implicated in various neural functions, and SNCA,a small pre-synaptic protein.Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation.Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1,thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts.CSK maintains LCK and FYN in an inactive form.Promotes CD28-induced phosphorylation of VAV1.
Function Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Cell membrane. Note: Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking.
Highlight Related Antibody Duos and Panels:
ARG30169 Src Family Protein Tyrosine Kinases Antibody Panel
Related products:
Fyn antibodies; Fyn Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cancer antibody; Neuroscience antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody
Calculated MW 61 kDa
PTM Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state (By similarity). PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling.
Palmitoylation at Cys-3 and Cys-6 regulates subcellular location.

Images (2) Click the Picture to Zoom In

  • ARG54079 anti-Fyn antibody ICC/IF image

    Immunofluorescence: HeLa cells fixed with -20°C Methanol and stained with ARG54079 anti-Fyn antibody at 1:50 dilution.

  • ARG54079 anti-Fyn antibody WB image

    Western blot: Jurkat cell lysate stained with ARG54079 anti-Fyn antibody at 1:500 dilution.