ARG55493
anti-Flotillin 1 antibody
anti-Flotillin 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Flotillin 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Flotillin 1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human FLOT1 (NP_005794.1) |
| Conjugation | Un-conjugated |
| Alternate Names | Flotillin-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FLOT1 |
| Gene Full Name | flotillin 1 |
| Background | Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq, Jul 2008] |
| Function | May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 47 kDa |
Images (2) Click the Picture to Zoom In
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ARG55493 anti-Flotillin 1 antibody WB image
Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG55493 anti-Flotillin 1 antibody at 1:1000 dilution.
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ARG55493 anti-Flotillin 1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55493 anti-Flotillin 1 antibody (green). Up: Primary antibodies. Bottom: Merge with DAPI (blue).
Customer's Feedback
Excellent
Review for anti-Flotillin 1 antibody
Application：WB
Sample：HeLa and 293T
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
