ARG62491
anti-Filaggrin antibody [FLG01]
anti-Filaggrin antibody [FLG01] for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human
Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [FLG01] recognizes Filaggrin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-Fr, IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | FLG01 |
| Isotype | IgG1, kappa |
| Target Name | Filaggrin |
| Immunogen | Recombinant full length protein. |
| Conjugation | Un-conjugated |
| Alternate Names | ATOD2; Filaggrin |
Application Instructions
| Application Note | FACS: 1µg for 106 cells IHC-P: 2 - 4 µg/ml IHC-Fr: 1/200 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FLG |
| Gene Full Name | filaggrin |
| Background | The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009] |
| Function | Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. [UniProt] |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 435 kDa |
| PTM | Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate. Undergoes deimination of some arginine residues (citrullination). |
Clone References
Highly rapid and efficient conversion of human fibroblasts to keratinocyte-like cells.
Papillary fibroblasts differentiate into reticular fibroblasts after prolonged in vitro culture.
IHC / Human
Effects of niacin restriction on sirtuin and PARP responses to photodamage in human skin.
IHC-P / Human
Characterization of a unique technique for culturing primary adult human epithelial progenitor/stem cells.
ICC/IF / Human
Human eccrine sweat gland cells can reconstitute a stratified epidermis.
ICC/IF / Human
Establishment and characterization of immortalized human gingival keratinocyte cell lines.
FACS / Human
