ARG64795
anti-Ferritin Light Chain antibody
anti-Ferritin Light Chain antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Ferritin Light Chain |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog |
| Tested Application | ELISA, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Ferritin Light Chain |
| Antigen Species | Human |
| Immunogen | C-GEYLFERLTLKHD |
| Conjugation | Un-conjugated |
| Alternate Names | NBIA3; Ferritin L subunit; Ferritin light chain; LFTD |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 20 kDa |
Images (4) Click the Picture to Zoom In
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ARG64795 anti-Ferritin Light Chain antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64795 anti-Ferritin Light Chain antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64795 anti-Ferritin Light Chain antibody IHC image
Immunohistochemistry: paraffin-embedded Human Brain Cortex (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64795 anti-Ferritin Light Chain antibody at 3.8 µg/ml dilution, followed by HRP-staining.
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ARG64795 anti-Ferritin Light Chain antibody WB image
Western blot: 35 µg of Human cerebellum (A), Human liver (B), Human placenta (C), Mouse brain (D) and Rat brain (E) lysates (in RIPA buffer) stained with ARG64795 anti-Ferritin Light Chain antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG64795 anti-Ferritin Light Chain antibody ELISA image
Sandwich ELISA: Capture rabbit antibody at 5 µg/ml dilution combined with ARG64795 anti-Ferritin Light Chain antibody as a detection antibody at 1.5 µg/ml dilution. Results of a typical standard run with optical density.
